Coding
Part:BBa_K4165083:Design
Designed by: Mennatallah Mahmoud Mohamed Abdelzaher Turky Group: iGEM22_CU_Egypt (2022-09-30)
SPINT3 (Serine Peptidase Inhibitor Kunitz type 3).
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 7
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 7
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 87
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 7
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 7
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was codon optimized to be expressed in E.coli
Source
Genomic sequence retrieved from the Uniprot